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Title: | Simple and sensitive HPLC-UV method for determination of bexarotene in rat plasma |
Authors: | Jong Bong Lee, Atheer Zgair Tae Hwan Kim, Min Gi Kim |
Keywords: | Bexarotene HPLC-UV preclinical pharmacokinetics rat plasma |
Issue Date: | 2016 |
Publisher: | Journal of Chromatography B |
Abstract: | Bexarotene is currently marketed for treatment of cutaneous T-cell lymphoma and there has been growing interest in its therapeutic effectiveness for other cancers. Neuroprotective effects of bexarotene have also been reported. In this study, a simple, sensitive and cost-efficient bioanalytical method for determination of bexarotene in rat plasma was developed and fully validated. The method utilises protein precipitation with acetonitrile and liquid-liquid extraction with n-hexane-ethyl acetate (10:1, v/v). An HPLC-UV system with a Waters Atlantis C18 column and a mobile phase of acetonitrile-ammonium acetate buffer (10 mM, pH 4.1) at a ratio of 75:25 (v/v), flow rate 0.2 mL/min was used. Chromatograms were observed by a UV detector with wavelength set to 259 nm. Intra- and inter-day validations were performed and sample stability tests were conducted at various conditions. The applicability of the method was demonstrated by a pharmacokinetic study in rats. Intravenous bolus dose of 2.5 mg/kg was administered to rats and samples were obtained at predetermined time points. As a result, pharmacokinetic parameters of AUCinf (4668 ± 452 h·ng/mL), C0 (6219 ± 1068 ng/mL) and t1/2 (1.15 ± 0.02 h) were obtained. In addition, the developed method was further applied to human and mouse plasma to assess the suitability of the method for samples from other species. |
URI: | http://localhost:8080/xmlui/handle/123456789/1045 |
ISSN: | 1570-0232 |
Appears in Collections: | كلية الصيدلة |
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File | Description | Size | Format | |
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lee2016.pdf | 435.42 kB | Adobe PDF | View/Open |
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