Mycosynthesis of Silver Nanoparticles using Terminia sp. Desert Truffle, Pezizaceae, and their Antibacterial Activity

Abstract

Desert truffle Tirmania sp. is appropriate as a greener reducing agent to synthesize silver nanoparticles at room temperature in a dark place by utilizing the aqueous extract of ascocarps of Tirmania sp. and 10P -3 PAgNOR 3R solution. Characterization of those nanoparticles was accomplished by changing color, UV-visible spectrum, FT-IR, AFM and SEM analyses. The changing in color is considered a first indicator for the formation of AgNPs which changed from pale yellow to dark brown after seventy-two hours. UV-visible spectrum showed the formation of AgNPs at peak 415 nm. AFM and SEM images demonstrated nanoparticles with spherical or irregular-shaped, agglomerated and highly heterogeneous. The higher zone of inhibition was 14 mm against Pseudomonas aeruginosa at a concentration of 5 mg/well while, the lower zone of inhibition was 9.5 mm toward Staphylococcus aureus at the level of 5 mg/well. These Tirmania-AgNPs had a promising antibacterial activity toward gram-negative and gram-positive bacteria, mainly the infectious bacteria of the eyes, Pseudomonas aeruginosa