Polymorphism of E-selection gene for coronary artery disease in Ramadi/Anbar - Iraq

Abstract

A lot of factors, such as genetic factors are contributing to coronary atherosclerosis. E- selection gene, which is also referred to as endothelial leukocyte molecule (CD62E or ELAM1), also the role of leukocytes and adhesion to activated endothelial cells is of high importance in the pathogenesis of coronary artery disease (CAD). The major goal of this work associating the mutation related to E-selection gene (Ser 128 Arg) with CAD in Ramadi / Anbar - IraqThere are 10 control subjects and 50 patients experiencing CAD are enrolled in the presented study. A561C polymorphism in E-selection gene through PCR succeeded via restriction fragment length polymorphism (PCR-RFLP). The frequencies related to CC, AC and AA genotypes have been (10%, 8%, and 82%) in patients experiencing CAD and (10%, 0% and 90%) in control subjects. There have been no considerable differences in genotypes' frequencies in S128R polymorphisms between control and CAD groups, (P= 0.499). Also, the frequency related to mutant C allele has been high in patients experiencing CARD in comparison to control group (5% vs. 14%). Yet, such differences weren't statistically significant (P = 0.186). Furthermore, the odds ratio with regard to CAD risks related to C allele is OR= 6.872; (95% CI=0.393 -120.017). The results indicated that genetic mutations in the E-selection gene at position 561 (Ser128Arg) do not correlate with the presence of the C allele in Ramadi / Anbar-Iraq.