Isolated and identification of mycosis infection associated with psoriasis

Abstract

At the AL-Ramadi Teaching Hospital in Al-Ramadi, seventy samples of hair, nails, and skin were collected from patients with probable dermatophytosis of various ages and genders. between the 8th of December and the 2nd of February (2020-2021). There were 32 males and 38 females among the 70 patients tested, with ages ranging from 8 to 76. The largest rate of dermatophyte infection was found in the age group 21-40, with 33 cases, followed by the age group 0-20, with 20 cases, and the age group 41-60, with 8 cases, while the lowest rate was found in the age group 61-80, with 3 cases (4.3 percent). All of the specimens To identify the dermatophyte species, they were examined directly (KOH 10%) and cultivated on Sabourauds dextrose agar media. In both males and females, direct microscopic inspection indicated positive results, and cases were verified by culture (containing dermatophytes and Candida spp.). Tinea pedis, Tinea capitis, Tinea corporis, and onychomycosis were the most prevalent clinical types. Tinea cruris and Tinea faciei are two species of Tinea that grow together. had the lowest infection rates because they were both. Males were found to have more T. pedis, T. corporis, T. cruris, and T. faciei, whereas females had more T. pedis, T. corporis, T. cruris, and T. faciei. were found in greater numbers in males. Six species from two genera were discovered among 26 isolated dermatophytes species. Trichophyton rubrum,, followed by ,Trichophyton verrucosum, ,was the most commonly isolated etiologic agent in the total. Other dermatophytes included Microsporum canis, Trichophyton interdigitale,, Trichophyton mentagrophytes,, and Microsporum gypseum,, in that order.The CTAB technique was used to extract genomic DNA from all isolated samples (dermatophytes and Candida spp.) with a purity of 1.7-1.8. To identify dermatophytes and Candida species, the ITS regions of all extracted DNA were amplified using the ITS1/ITS4 primer combination. The goods were excellent. Electrophoresed 2 percent agarose gels in TBE buffer were electrophoresed, and the results were visualized using ethidium bromide staining under UV irradiation.