Microbial Technique for The Production of Growth Regulator Compounds Indole Butyric Acid (IBA) and Its Role on The Rooting and Growth of Bougainvillea Spectabilis L. Stem Cuttings

Abstract

The study included the application of microbial techniques to convert sesbanin and soybean seed powder into indole bio-compounds by using 3 bacterial isolates: Azotobacter chroococcum, Pseudomonas putida and Bacillus.megaterium obtained from the microbiology laboratories at the Center for Desert Studies. Peptone 1%, Then it was used alone, in pairs, or in combination to produce the indole butric acid (IBA) growth regulator compounds, using a medium containing locally prepared materials that included the dry powder of soybean seeds and sesbanin seeds. It also tested the ability of isolates to dissolve phosphate compounds and produce iron-chelating compounds. The results showed the ability of B. megaterium A. chroococcum isolates together to produce indole compounds at a rate of 182.3 mg L-1 when using a mixture of sesbanin seed powder and soybeans. The highest product rate of bio-indole compounds was 67.9 mg L-1 with the use of a mixture of soybean seed powder and sesbanin medium.While the highest product rate reached 103.33 mg L-1 by using the two isolates mixture of B. megaterium and A. chroococcum together. Also, P. putida, B. megateriumand, A. chroococcum isolates were found are able to produce iron-chelating compounds (+++, ++, ++) according to the sequence. These isolates B. megaterium , P. putida and A. chroococcum were found ability to dissolve phosphate compounds with average dissolving diameter of 10.43, 29.9 and 29.6 mm, respectively. The ability of the Bio-IBA growth regulator product was tested with or without cells isolates compared to industrial IBA at concentrations of 300 and 600 mg L-1 to influence some growth cutting of Bougainvillea spectabilis L. The results showed a significant effect on some growth characteristics such as germination percentage, number and area of leaves per plant, branch length and number of branches of the plant.