Effect of Steroidal Extract for Fenugreek Seeds on ICSH and SSH in Albino Male Mice

Abstract

Plants are used to treat many conditions of diseases but have many side effects which lead to deleterious effect for several systems in the body. The objectives of this Study are to investigate effective role for steroidal extract of fenugreek on ICSH and SSH in male mice hormones. The steroidal extract of fenugreek was prepared by hydrolyzing the grinded powder of fenugreek seeds with HCl (2N) in water bath, then filtered and air-dried and put in soxhlet apparatus for 16 hrs with addition of chloroform as a solvent; the solution was condensed in the incubator and preserved in refrigerator until use. Thirty (30) adult Albino male mice of 8-10 weeks old, their weight ranged between 25-35 gms. they were randomly divided into 3 equal groups, 10 mice for each group. The steroidal extract of fenugreek seeds administered at dose (100, 200) mg/kg B.Wt. to first 2 groups (G1 and G2) respectively orally for 34 days, while the third group (Control) administered daily ethyl acetate 20% orally. The results of the present study demonstrated significant decrement (P<0.05) in both G1 and G2 mice in all parameters measured (ICSH and SSH hormones) as compared with G3 (control group). It was concluded that the steroidal extract of fenugreek seeds has a significant antifertility effects on male reproductive mice and this effect increase with increasing in dose of this extract.