Some Studies on leaves Extracts of Olive and Morus alba in experimentally STZ induced diabetes in rats

Abstract

The aim of this study is to investigate the effects of Olive and morus alba leaves extracts(ethanol hydro-alcohol 60%) on anti-oxidant parameters {superoxide dismutase (SOD), glutathione peroxidase (GSH), Catalase (CAT) and Malondialdehyde (MDA)} and on lipid profile {Cholesterol (Cho), Triglyceride (TAG), HDL, LDL and VLDL} in experimentally STZ induced diabetic in rats. Fifty adult male albino rats (weighting 150 -200 g) were used and divided into 5 experimental groups (10 rats in each group). The first group was served as control group. The remaining groups were injected (i.p) by streptozotocin (STZ) at 45 mg/kg b.wt to induce diabetes. The 2nd diabetic group was left as control diabetic group. The third diabetic group was treated with Cidophage (500 mg/kg, orally).While, the fourth and fifth diabetic groups were treated with Olive leaves and Morus alba leaves extracts (500 mg/kg b.wt, 600 mg/kg b.w respectively orally), respectively. All treatment was given daily for successive 30 days. The levels of some anti-oxidant parameters (SOD, GSH, CAT and MDA) and lipid profile (Cholesterol, TAG, HDL, LDL and VLDL) were measured at 1st day, 1st and 2ndweeks after treatment. The rats were sacrificed at the end of 30 days post treatment. The obtained results demonstrated that, the use of Olive leaves and Morus alba leaves extracts improved the levels of the tested parameters in diabetic rats. Hydro-alcoholic leaves extracts of Olive and Morus alba could improve the anti-oxidant and lipid profile parameters of male rats, and minimized the possible side effects of diabetes mellitus.