Green Synthesis of Gold Nanoparticles using Pineapple Extract and Study their Analytical Characterization and Antibacterial Activity

Abstract

Many methods were used to synthesize nanomaterials but, in this study, we used a simple and ecofriendly method for preparation of gold nanoparticles (AuNPs) called the green method. This method was rapid and cheap. Some factors were affected on the preparation of AnNPs such as the temperature and the concentration of Au ions. the color of the solution was ruby red because of formation AuNPs, and gave absorption peaks between 520-540 nm. TEM images contributed by studying morphology of AuNPs. The shape was almost spherical and its size ranged between 20-70 nm. Stability of Au NPs was investigated by measuring the absorption spectra of the gold colloidal sol prepared at various times. This work involved study of amikacin, Trimetho, Doxycycline and Gentamicin against E. coli bacterium. We noticed increasing in inhibition zones area from 11341.6 mm2to 2207.2 mm2, 616 mm to 1964.4mm2, respectively when we used synergistic effect of Au NPs with antibiotics..