Biosynthesis, Characterization and Cytotoxicity of Zinc Nanoparticles Using Panax ginseng Roots, Araliaceae

Abstract

This study aims to biosynthesize zinc nanoparticles from the aqueous extract of Panax ginseng (Red Ginseng) roots. The characteristics of ZnNPs were checked using change in color, UV-Vis, SEM, SPM, AFM, FT-IR, and EDS analyses, and as sessed their cytotoxicity against L20B tumor cell line using MTT assay. The change in the solution color after 3 hr on 70 °C is from yellow to the brownish color with whitish sediments. The adsorption peak of UV-vis is 340 nm as evidence of forma tion the Zn nanoparticles. AFM, SEM and EDS observe shapes of zinc nanoparti cles which are spherical to irregular particles with rate of size 59.76 nm. The con centration 100% Zinc nanoparticles significantly (p<0.01) recorded best inhibition percentage 41.70% against murine fibroblast cells (L20B) which have receptors of human polioviruses. Thus, this work is considered as an auspicious first test to re duce the growth of cancers using green ZnNPs of Ginseng (Panax ginseng) in vitro