Mycosynthesizing and characterizing silver nanoparticles from the mushroom Inonotus hispidus (Hymenochaetaceae), and their antibacterial and antifungal activities

Abstract

The current research aims to produce silver nanoparticles from isolated Inonotus hispidus (Shaggy Bracket fungus) locally from Hit, Iraq. The silver nanoparticles were mycosynthesized from the mushroom extract by adding 10−3 M AgNO3. The properties of Ag nanoparticles were studied by UV–vis, XRD, FTIR, AFM, and Zetasizer. The color changed from light yellow to brown after 90 min, and the lambda max was at 410−420 nm by the UV–vis spectrum. Zetasizer showed the formation of AgNPs with an average of 69.24 nm. Results of the XRD show that AgNPs have a cube-centered crystalline face. The FTIR showed that active groups belonging to the carbonyl group (C]O) in the form of amides (HNeC]O) in peptides and proteins, as well as the presence of this group in flavonoids, polysaccharides and phenolic compounds. The concentrations 75% and 100% of AgNPs exhibited zone of inhibition 10 mm and 11 mm, respectively against Escherichia coli. The lowest zone of in hibition was 7.5 mm toward Klebsiella with the concentration of 50%. Also, the higher inhibition percentage against fungi was 12.2% against Aspergillus niger, whereas the lower percentage was 6.3% against Aspergillus flavu. This paper is considered the first attempt to synthesize AgNPs from Inonotus hispidus, which applied against bacteria and fungi