The Effect of Strontium on Osteoblastogenesis and Osteoclastogenesis in Dental Stem Cells-induced Epidermal Growth Factor at Molecular Level: In Vitro Study

Abstract

Abstract: For a reconstructive surgeon in oral and maxillofacial surgery, repair of massive bone defects in craniofacial region and mandible caused by different types of trauma, extensive bone destruction by cancer or metabolic diseases is amongst one of the most difficult tasks to handle. The aim of this study was to assess the effectiveness and potential effect of incorporating Strontium (Sr) with locally delivered epidermal growth factor (EGF) on osteoclastogenesis and osteoblastogenesis gene expressions of dental stem cells (DSCs) in an in vitro study. Sr, EGF, and dental stem cells (DSCs) with all materials (reagents and drugs) were commercially purchased from companies. Viability test (cytotoxicity test) was carried out to determine compatibility and optimal concentrations of Sr scaffold and EGF in accordance with the protocol of the current study. DSCs were treated with three modalities of drugs base time points, control-0 concentration, DSCs treated with Sr only, DSCs treated with EGF only DSCs treated with Sr/ EGF. RNA from DSCs were extracted from all treated groups. RT-PCR was used to amplify the specific osteoblast/ osteoclast genes markers. Electrophoresis of the RT-PCR products was followed by gel image capturing. Significant enhanced osteoblast markers responsible for bone healing were observed in the DSCs group that were treated with Sr/ EGF as compared with the other studied group. This strategy seems to be a reliable new tool for bone tissue engineering by mean using bone graft material potentiates inducing high capacity of bone wound healing and could be effective strategy in reconstructive surgery when used in in vivo study used stem cellsbased therapy