Studing the effects of aquatic extracts of Nigella sativa, Olea europacea and Allium sativum on shigella dysenteriae in vitro

Abstract

Three aquatic extracts of Nigella sativa, Allium sativum and Olea europacea were used to determine their effect on shigella dysenteriae that was isolated from a stool of patient with diarrhea. Filter paper disks were prepared to socked with plant extracts to be used on Muller Hinton agar plates that were inoculated with the bacteria. After 18-24hours, inhibition zones were measured. Nigella sativa extracts were found to be the best since the mean inhibition zone was 23 mm followed by Olea europacea extract which showed mean inhibition zone was 22mm, while the lowest inhibition zone diameter was noticed in Allium sativum 16mm. These three medicinal plants were chosen because they were known to contain some elements that are effective against enteric bacteria.