Anti-diabetic effect of β-amino Butyric Acid in Streptozotocin induced Rats

Abstract

Aim: Using Beta amino butyric acid (BABA) in experimental induced diabetes mellitus male Sprague Dawley rats (Rattus norvegicus domestica) by Streptozotocin (STZ) injection. Materials and Methods: Diabetic rats received intraperitoneal injection of BABA (100 or 200 mg/km body weight /d) daily for 3 weeks. After the end of the trial period, blood samples were collected and serum was isolated to measure the concentrations of glucose, insulin, MDA, SOD. In addition, a comet assay was performed for hepatocytes. Results: Diabetes results in a significant elevation in glucose, MDA levels and increase percentage of high damage for DNA in comet assay. The results also revealed a significant decrease in insulin levels, SOD as well as a decrease in non-damaged DNA percentage in the comet assay, at the probability level (P< 0.05). While the treatment with the non-protein amino acid BABA in both concentrations lead to a significant decrease in the levels of glucose, MDA in addition to a reduction in percentage of high-damaged DNA in comet assay in addition to a significant elevation in the level of insulin, SOD and rise in non-damaged DNA percentage in the comet assay (P< 0.05). Conclusions: Our study revealed BABA would be useful for diabetes therapy through improving of impaired glucose metabolism and recovering oxidative stress. It also may have antioxidant properties likewise results indicate that the preventive effects may inhibit lipid peroxidation as a result of its antioxidant nature. also indicated that BABA was able to reduce DNA damage in male diabetic rats' liver.