Effects of In-Ovo injection of Biotin on chick’s embryonic development and physiological traits

Abstract

This study was conducted at the local hatchery in Ramadi, Anbar Iraq from 1st to 23th Dec. 2018, to 26 Feb 2019. The objectives of this study were to investigate the effect of injected eggs hatching in times and different concentrations of Biotin in growth and embryonic development, hatchability. Six hundred eggs of hatching types (Ross 308) and injected with different concentrations of biotin at age of 0 days (before placing in the hatchery) and 18 days of incubation. Eggs were divided into five groups (120 eggs for each) as follows: 1.T0: Control group placed in the hatchery without injection. 2.T1: Injected with a dose of 100 μg biotin at age of zero. 3. T2: Injected with dose 75 μg biotin at age zero. 4.T3: Injected with a dose of 100 μg biotin at age 18 days of incubation. 5.T4: Injected with a dose of 4175 μg biotin at age of 18 days of incubation. Statistical analysis was performed (CRD) (P=0.065) results show: Increase length of the embryo, diameter of vascular region and number of pairs of somites at 3 days of incubation for T2. Increase percentage of embryonic weight, decrease the percentage of albumin and the percentage of at 7 days of incubation for T1 and T2. Increase in percentage of embryonic weight and amniotic sac and liquid, decrease in the percentage of albumin and yolk, at 14 days of incubation for T2. Increase percentage of embryonic weight, decrease the percentage of yolk at the age of 17 days incubation for T2. Increase hatchability of total eggs and fertile eggs. Decrease embryonic mortality and different stages of embryonic development. Concluded that In-Ovo injection of the hatching eggs with biotin contributed to increase physiological traits and embryonic development.