PHENOTYPE AND MOLECULAR STUDY FOR SOME BACTERIAL ISOLATES WHICH ISOLATED FROM DIARRHEA PATIENTS IN RAMADI CITY

Abstract

This study included collected 280 stool samples from patients with diarrhea in the Ramadi Teaching Hospital and the Women’s and Children’s Hospital to Phenotype and Molecular study. For some bacterial isolates which isolatied from diarrhea pateints DNA was extracted then amplified the LT1, ST1, set 1 and stn genes by polymerase chain reaction through specific primers. The results of positive bacterial culture were 200 bacterial isolated included E. coli (77.5%), Salmonella typhimurium (5%), S. muenchen (2.5%), Shigella flaxneri (5%), Klebsiella (4%), Pseudomonas (3.5%) and Proteus (2%). The results of PCR reveled the LT1, ST1 gene were found in E. coli isolates with the presence of LT1 gene (85.7%), while ST 1 was not found in E. coli isolates. The results of the Stn gene analysis were found for all isolates and both S. typhimurium and S. muenchen in 100%. The results of the detection of the set1 gene for Sh. flaxneri showed contained all isolates set1 gene (100%). The PCR products were sequenced and detected variation compare with data base in NCBI by using the MEGA7 program, the results for DNA sequencing for LT1 and ST1 showed similarity 100% with NCBI except single tranisition (A-G) in position 233. The results for stn gene appear transition polymorphism (T-C, G-A and A-T9) in position 609, 565 and 571, respectively. And transversion (A-T), whereas the result for set1 gene showed 100% similarity with database with single transition (T-C) in position 76.