DETERMINATION OF GENETIC DISTANCE AMONG GENOTYPES OF BREAD WHEAT TRITICUM AESTIVUM L., USING ISSR MARKERS

Abstract

This research was conducted in biotechnology laboratories of grain test and validation office-Ministry of Agriculture in 2017. Fourteen wheat genotype that represented pure local and imported classes, were cultivated to evaluate genetic diversity and determine genetic affinity among them by using 12 primer inter Simple Sequence Repeats (ISSR), 11 of showed an activity to give a polymorphism among the studied genotypes which give 118 alleles, these primers ratio of this polymorphism was 89. The primer UBC827 gave higher bands of 17, while the primer A33 gave lower bands of 5. The study showed that genetic similarity of the genetic affinity ranged between 0.174 – 0.843 in which the higher genetic affinity (0,843) was for V1 and V2 varieties followed by (0.680) for V1 and V3 varieties, The lower genetic affinity (0.174) was for V10 and V11 varieties.