Molecular study of some Virulence Factors Enteropathogenic Escherichia coli isolated from newborn till the age of one year

Abstract

Collected 240 samples from Abu_Ghraib Teaching Hospital, included 120 samples of Stool, 120 samples of urine, for a period from January 07, 2017 to April 07, 2017.The strains of E. coli samples were examined in the central laboratories of health in Baghdad, the examination was conducted using polyspecific test reagents and mono specific test reagents. The results polyspecific test reagents show that the dominated serotype is Enteropathogenic E. coli for ages 1 day to 1 year. The results of mono specific test reagents show that the percentages of serotype are: O103 (25,7%) overall and; 60% in stool and 40% in urin, O26 (20.57%) (61%S,39%U), O78(18.85%) (54.5%S,45.5%U), O25 (12.57%) (50%S,50%U), O118 (10.85%)(53%S,47%U), O142 (8.57%) (47%S,53%U). The resistance results of the bacterial sensitivity tests for 15 antibiotics on the E. coil were: Penicillin G (100%), Erythromycin (99.5%), Tetracycline (99.5%), Cephalothin (99.4%), Clindamycin (99%), Trimethoprim (98%), Amoxicillin (90.9%), Cefotaxime (89.7%), Chlorphenicol (79.4%), Ampicillin (63.4%), Netimicine (38.9%), Nalidixc Acid (33.7%), Norfloxacine (12%), Ciprofloxacine (10.9%), Tigecycline (0%). 30 isolates were selected for E. coli strains which included 20 stool isolates and 10 urine isolates to examine the presence of 6 virulence genes using the polymerase chain reaction technique (PCR), The percentages of virulence genes were: eaeA gene (63.3%), Stx_1 gene (100%), UidA gene (96.66%), neuC gene (23.3%) and hlyA gene (80%). The bfp gene was not obtained in the study samples because it is a diagnostic gene.