Water, Alcoholic and Etheric Extraction Method for Some Active Materials from Linden Plant, So Antibacterial and Antitumor Activity Studies

Abstract

The aim of this study is to isolate some active substances from the linden plant (Tilia cordata) as tannins and saponins and volatile oils. The extraction method shows the percentage of the isolated materials was ((31.1%) (59.2%) and (9.7%) respectively, as well as the appointment of some mineral elements in the linden as sodium, calcium, potassium,was (ppm 86), (ppm 65), (ppm 467), respectively,by application atomic emission spectroscopy. In addition study effectiveness of the anti-bacterial plant extractants of linden using two types of pathogenic bacteria it (Escherichia Coli and Staphylococcus aurous), where the study showed the ability of inhibition of the different extractants and different inhibition diameters vary according to different active substances and concentrations and type of the bacteria. Used one type of cancer cell lines to study the effect of extracts of lime on the growth of cells in the laboratory and thus know the specifications of the extractants anti tumor cell line used is (L20B) a mutant mouse cells (Mice Transformed cell Line)