Moringa Extract Protects ALAD Gene Expression in Rats against Pb-Heavy Metal Poisoning

Abstract

This study investigated the possibility of using Moringa oleifera extract to prevent poisoning caused by commercial lead nanoparticles (C.) and induced by pulsed laser ablation (L.) in white Wistar rats. The rats were divided into six groups with seven rats in each group: (1) 160 mg kg-1 Moringa seed extract (M.), (2) 5 mg kg-1 commercial lead nanoparticles(C), (3) 5.8 mg kg-1 nanoscale lead(L), (4) Moringa seed extract + commercial lead nanoparticles (C. M.), (5) Moringa seed extract + prepared lead nanoparticles (L.M.) and (6) control(Con.). After exposure for 45 days, blood was drawn from the animals and the genetic changes of the ALAD gene were studied. Moringa seed extract (160 mg Kg-1) was efficient to protect ALAD gene expression which gave 1.073725, followed by control of 1 and nanoscale lead X Moringa seed extract (5.8 X 160 mg Kg-1) 0.421775. principal component analysis, spider (radar) and circular analysis were very informative to extract the variance resulted from treatments on ALAD gene expression.