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DC Field | Value | Language |
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dc.contributor.author | Omar1, A. A. | - |
dc.contributor.author | Ahmed2, M. N. | - |
dc.contributor.author | Asker3, Audai Sabah | - |
dc.contributor.author | Majeed1, A. F. | - |
dc.contributor.author | Faraj4, T. M. | - |
dc.contributor.author | Rejah41, S. J. | - |
dc.date.accessioned | 2022-10-20T19:31:25Z | - |
dc.date.available | 2022-10-20T19:31:25Z | - |
dc.date.issued | 2021-03-02 | - |
dc.identifier.uri | http://localhost:8080/xmlui/handle/123456789/4156 | - |
dc.description.abstract | AbstractThis study was undertaken to show the effect of follicular size and culture media on the results of IVF and IVEP in sheep. A hundred ovaries of slaughtered ewes were taken during non-breeding season. The total number of follicles were 256, while the number of recovered oocytes were 189. The recovery rate of oocyte from large and small follicles were 85.8% and 64.7% respectively. Only grades A and B oocyte have been used with a total number of 148 oocytes. Maturation and fertilization rates in different media; MEM, RPMI1640, DMEM low glucose and DMEM high glucose were 64.4%, 51.2%, 32.4% and 26.6% for maturation rate and 66.6%, 55%, 50% and 37.5% for fertilization rate respectively. There was a statistical difference (p≤0.05) in maturation and fertilization rate between different media; the total fertilization and cleavage rate was 56.7% and 70% respectively. The numbers of morula and blastocyst stage were 40% and 35% respectively. There was a statistical difference (p≤0.05) in morula and blastocyst production in different media. It was concluded that abattoir was a good source of oocyte recovery, and the large follicle give the best morula and blastocyst stage. MEM was a good diluent of semen. mMEM give the best result as cultured media as compared with the other media used in this study | en_US |
dc.language.iso | en | en_US |
dc.subject | IVF, | en_US |
dc.subject | IVEP, | en_US |
dc.subject | Local Iraqi breed sheep Non-breeding season | en_US |
dc.title | In Vitro Production of Ovine Embryo in Non-breeding Season | en_US |
Appears in Collections: | قسم الانتاج الحيواني |
Files in This Item:
File | Description | Size | Format | |
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In-Vitro-Production-of-Ovine-Embryo-in-Non-breeding-Season14.pdf | 643.48 kB | Adobe PDF | View/Open |
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