Role of Nitroglycerine Injection in Japanese Quail (Coturnix japonica) Testes Tissues Parameters

Abstract

Abstract: This study aimed to evaluate injection Japanese quail males Coturnix japonica with nitroglycerine NTG subcutaneous in testes tissues parameters. 45 males of Japanese quails used in this study divided into 3 treatments, 3 replicate. Males injected subcutaneous one time weekly for six weeks by 0.5 ml of 2.5 and 5 mg of NTG dissolved with sesame oil while control group left without injection. Males slaughtered and testes weight, testes relative weight, seminiferous tubules, and interstitial cells volume density and relative weights (mg/g) and contents diameters used in this study. Results show that significant increasing (P≤0.003) in volume density percentage of spermatogonia and significant decreasing (p≤0.05) in relative weight of Sertoli cells for 2.5 mggroup and significant increasing (P≤0.03) in Sertoli cells volume density percentage for 5 mg group compared with control group. Significant decreasing (p≤0.05) in relative weight of basement membrane, -1 significant increasing (P≤0.0001) in seminiferous tubules diameter and significant decreasing (p≤0.009) in testes relative weight (g kg ) and total seminiferous tubules contents for all treatments compared with control group.