Study the Synergistic Effect of AgNPs with Antibiotics on Klebsiella Pneumoniae Isolates Which Diagnosed by Molecular Methods

Abstract

The current study was conducted to study the synergistic effect of AgNPs with anti-biotics on Klebsiella pneumonia isolates, where 50 samples were collected from different pathological conditions (wounds - burns - urine - ear - and excretion) on the Maconkey Agar . To the Klebsiella , it was diagnosed microscopically and biochemically and was also diagnosed by the Vitek2 device, whose results were identical with the biochemical diagnosis, and the isolates were also subjected to molecular diagnosis for confirmation by using the PCR technique based on the diagnostic gene 16SrRNA, which is characterized by being good, stable and low heterogeneity for a long time in the bacterial type Ten types of antibiotics were selected and immersed in silver nanoparticles At a concentration of 20Mm, a test was conducted for the synergy between the silver nanoparticles AgNPs and the antagonists towards 12 isolates of Klebsiella bacteria, as they were applied to Muller-Hunton medium and after they dried up, the anti-saturated tablets were fixed with a silver particle solution, and after the expiration of the incubation period, the diameter of the inhibition area around the tablets was measured in mm According to the standard results, the results of the statistical analysis showed that the interaction of silver nanoparticles with the antibiotics had a significant effect on increasing the sensitivity of the isolates towards the synergistic antagonists, as the general rate of inhibition diameters of the antagonists (Impenem, Gentamicin, Amikacin Ciprofloxacillin) was (32.59, 21.6, 18.98, 36.18), respectively. , This activity is due to the ability of the nanoparticles to bind to the surface of the bacterial cell The binding causes structural damage and changes such as permeability, which facilitates the entry of the antagonist into the cell and thus its death. As for the synergies with the antagonists Tetracycline, Vancomycin, Erythromycin did not show efficacy in the direction of Klebsiella isolates did not give good results of synergy as it has a weak effect towards negative bacteria and it is as a non-synergistic antagonist because it does not It has effective binding sites for silver nanoparticles