EFFECT OF SUPPLEMENTATION OF ZINC AND VITAMIN-E AND THEIR COMBINATION ON PHYSIOLOGICALTRAITS OF BROILER CHICKENS UNDER OXIDATIVE STRESS CONDITION

Abstract

Abstract This study was carried out to evaluate the effect of adding zinc and vitamin E and their combination to the broiler chicken diet on physiological traits of broiler chickens under oxidative stress condition. Oxidative stress was introduced by adding hydrogen peroxide (H2O2 ) to drinking water from the age of 10 days with 0.5% for all treatments except the negative control treatment (T1). Two hundred eighty two, unsexed one day old broiler chicks (Ross 308) were reared at average body weight (BW) of 38 g, were randomly allotted into 8 treatments with 3 replicates per treatment (each replicate represents one pen) and 12 birds per replicate for 42 d. The treatments were included T1 free of any addition (negative control), T2 basal diet + 0.5% of H2O2 (positive control), T3 basal diet+ 80 of mg/kg of vitamin E, T4 basal diet + 160 mg/kg of vitamin E, T5 basal diet + 40 mg/kg of zinc, T6 basal diet + 80 mg/kg of zinc, T7 basal diet + 40 mg/kg of zinc + 80 mg/kg of vitamin E, and T8 basal diet + 80 mg/kg of zinc + 160 mg/kg of vitamin E. The obtained results of blood characteristics of broilers in 42 days showed insignificant differences between different experimental treatments in the value of hemoglobin and the number of red blood cells while T6 was significantly (P <0.05) exceeded in the percentage of hematocrit compared to all experimental treatments. In regard to the number of white blood cells, the percentage of heterophils, rate of sedimentation velocity of blood cells, it was observed a highly significant (P <0.01) in T2 compared to all experimental treatments and with respect to the percentage of lymphocytes T6 treatment was recorded a highly significant (P <0.01) compared to all experimental treatments, as well as for the characteristics ofbiochemical blood plasma, T2 recorded a highly significant (P<0.01) in the glucose of blood plasma compared with other experimental treatments in addition to a highly significant (P <0.01) in T1 in the total protein rate was observed which was not significantly different with T5, T7 and T8. In terms of the albumin level, it was highly significant (P <0.01) in T2 compared to all experimental treatments, while the level of globulin was significantly (P<0.05) exceeded in T1 compared to T2 and T6. For the level of cholesterol, T2 was significantly exceeded compared to the other treatments, and with regard to the level of zinc, T8 did not differ significantly with T1, T3 and T6. In relation to the efficacy of the AST enzyme, T2 was significantly (P<0.05) exceeded compared to other treatments, and with regard to the effectiveness of the enzyme ALT a significant decrease (P<0.05) in T2 compared with other experimental treatments was observed. Additionally, T2 showed a significant increase in the level of Malondialdehyde (MDA), the Peroxide Value (P.V) and Free Fatty Acid (FFA) in liver tissue compared to the other treatments.