EFFECT OF SUPPLEMENTING EXTENDER WITH MELATONIN ON IRAQI NATIVE SEMEN QUALITY DURING COOLING STORAGE

Abstract

ABSTRACT : This study aimed to evaluate supplementation of Melatonin to Lake 1960 chicken semen extender and semen liquid storage under 5 C to semen quality. In this study, 20 fertile Iraqi native roosters used. Semen collected from all roosters (pooled sample) in a glass tube for 3 times, 3 days per another one. Semen divided into 4 tubes and diluted 1:1 by using Lake 1960 extender with 0, 0.5, 1 and 1.5 mM/L melatonin to the extender, then stored under 5C in glass tubes for 0, 12, 24, 48, 72 and 96 hours. Sperms individual motility, viability, abnormality, and acrosomes abnormality evaluated for each treatment and time storage. The results showed that decreasing (Pd≤0.0001) in sperms individual motility for all Melatonin treatments compared with control groups at 96 hours. No significant differences between Melatonin treatments and control for another storage times. Decreasing (Pd≤0.05) in sperms abnormality in 1.5 mM/L Melatonin for 96-hour storage compared with other groups. Decreasing in acrosomes abnormality (Pd≤0.05) in 1.5 mM/ L Melatonin for 24- and 48-hours storage. Moreover, Melatonin supplementation to semen extender improved sperms motility and decreasing sperms and acrosomes abnormality.