Comparative Evaluation Of Effect Of Irrigants On The Mechanical Properties Of Dentin – An In-Vitro Study

Abstract

Aim: The present study aimed to evaluate the effect of irritants:sodium hypochlorite (NaOCl), Ethylene diamine tetra acetic acid (EDTA), chlorhexidine (CHX) and chitosan nanoparticles (CNP) on the modulus of elasticity and flexural strength of radicular dentin. The null hypothesis was that there will not be any difference among the tested irrigants. Materials and methods: Seventy-five dentine bars were prepared from orthodontically extracted premolars and stored in deionized water. The samples were divided into 5 experimental groups (n= 15 per group): Group 1 - 2.5% NaOCl, Group 2 - 17% EDTA, Group3- 2% CHX, Group 4 - 0.2% CNP, Group 5 - Saline (Control). The samples were immersed in 24 well tissue culture plates containing one of the solutions for 30 minutesand rinsed with deionised water.They were then subjected to three-point bending test. The samples were placedon the Instron universal testing machine and loaded with a cross headspeed of 1 mm/min until fracture occurred.The modulus of elasticity and flexural strength were calculated accordingly. The values were statistically analysed using one-way analysis of variance (ANOVA) and Fisher’s test for pair wise comparison.