Effect of arginine and selenium with vitamin E in some biochemical properties of Inseminated ewes

Abstract

The study was conducted in one of the fields of the private sector within Basaaer the village of the district of Hit / Anbar at 180 km west of Baghdad, during the period from 17/4/2018 to 30/5/2018. 28 ewes with an age of 2-4 years with a weight of 40-50 kg with at least one birth. All ewes were tested using ultrasonography to ensure that they were free of pregnancy before the start of the experiment. Synchronization of estrus with intravaginal sponges was achieved in ewes, and inseminated with rams at the end of the hormonal program. The rams remained with the females for five days. The ewes were randomly divided into four equal groups (7 ewes in each group). The first group were injected with 160 micro mol / kg body weight and from the fifth day of the inseminated until day 20 of pregnancy The second group was treated by injecting selenium plus vitamin E with (2 ml/head α-Tocopheryl Acetate 68mg/ml Selenium 1.5mg/ml) intramuscular. The first dose was given on day 5 and the second one on day 19 of insemination the early at pregnancy. The third group were injected the arginine with the selenium plus vitamin E emulsion, The fourth group was injected with a physiological saline solution and considered as control group. Blood samples were collected from jugular vein prior to treatment on day 4 of the insemination as well as on days 10, 15, and 18, to measure the changes in the chemical properties at the early of pregnancy. The results of the study showed a significant difference (P≤0.05) between the treatments and control groups. The results showed a decrease in the concentration of urea and ALT enzyme as compared with the first group and control. The present study did not show a significant differences at early of pregnancy in the concentration of cholesterol, total protein, albumin, globulin, glucose, and AST enzyme at the early of pregnancy for ewes. The results also showed a significant difference (P≤0.05) between the different withdrawal periods within the same treatment in the control and other groups the concentration of ALT enzyme, and total protein. It can be concluded from this study that the use of arginine and selenium with vitamin E improves maternal health by reducing the level of urea and ALT enzyme and maintaining pregnancy after insemination.