Effect of Acridine Orange in Promoting Growth and Physiological Characteristics of Fragaria Ananassa Duch Under Salinity Stress in Vitro.

Abstract

Abstract. This experimental were conducted out at plant tissue culture lab., Center of Desert studies to test the effect of four concentrations of Acridine Orange viz., 0 , 10 , 50 and 100mM L-1 under four levels of salinity viz., 0 , 30 , 60 and 90 mM NaCl L-1 on some phenological traits including: leaves number, branches number, plant length and fresh weight. Membrane stability index and electrolytic leakage also were in vitro measured. The experiment was factorial and arranged in a complete randomized design (CRD) with five replicates. Results revealed that 50 mML-1 of Acridine orange gave highest leaves number of 14.67 leaves explant-1, branches number of 3.75 branches explants-1, plant length of 1.48cm and fresh weight of 0.71g. whereas, 10 mM L-1 membrane stability index of 76.63. Furthermore, 30 mML-1 possesses highest leaves number of 11.75 leaves explants-1, branches number of 2.83 branch explant-1 , plant length of 1.29cm and fresh weight of 0.58g. The highest level of salinity (90 mM) gave highest of electrolyte of 69.07.It could concluded that 50 m mol l-1 of acridine orange was the best concentration to promote growth of strawberry in vitro. Moreover, Plantlets were more tolerant to 30 m mol L-1 of Nacl in vitro.