Effect of recovery methods of bovine oocytes on in vitro maturation

Abstract

Abstract This study was designed to compare between two methods collecting oocytes (slicing - ORCS) and its effect on maturation of oocytes in vitro. oocytes of cows were harvested using OCRC and slicing methods from gental organs in slaughter house. used media culture SOF for maturation oocytes (at 38.5 ͦC, 5% CO2 and 90% relative humidity for 24 hrs..( The results showed significant difference (P<0.05) in maturation rate between oocytes collected by slicing effect on maturation oocytes in vitro cows 57.76 % as compared with ORCS method 43.54 %. In conclusion, there is a difference for the maturation oocytes in vitro using bovine two methods slicing compared with ORCS, but these methods are encouraged to get a large number of oocytes, which encourages Bmdaolentha for vitro purposes (cloning, sexing manipulation of embryos)