Effect of Natural Antioxidants in Some Blood Parameters and Antibodies Titer Against Newcastle Disease in Heat Stressed Brown Laying Hens

Abstract

Abstract This study was study was carried out at the Poultry Research Station belong to State Board of Agricultural Research (Ministry of Agriculture, Abu- Ghraib). The study lasted for 12 weeks (84 days) from summer season, 200 Lohman brown layer (34 weeks of age) were randomly distributed into 5 treatments, with two replicate per treatment (20 hens per replicate), the first treatment was the control group, the hens in 2nd and 3rd treatments were given vitamin E and C 300 mg/kg feed respectively, the 4th and 5th groups of hens were given grape seed and rosemary 5 g/kg feed respectively, poultry house temp. 36 cº. The results revealed that adding natural antioxidant (grape seed and rosemary leaf) significantly increased of glutathione concentration in liver tissue of natural antioxidant compared with the artificial antioxidant and control treatment and adding natural antioxidant significantly increase the PCV percentage, Hb concentration in the blood, also, whereas, significant decreased in the WBC and H/L ratio in the blood of hen consumed diet having adding natural antioxidant significantly increase the PCV percentage, Hb concentration in the blood, also, whereas, significant decreased in the WBC and H/L ratio in the blood of hen consumed diet having antioxidants compared with the control treatment and adding vitamin E were significantly increased antibodies titer Newcastle Disease compared with the all the treatments.