Assessment of Salicylic Acid Capability in Biofi lm Disassembling of Local Penicillin Resistant -Streptococcus Agalactiae Isolated from Pregnant Women

Abstract

Streptococcus agalactiae, is the main aetiological agent of early neonatal sepsis in developed countries transmitted from pregnant women.Twenty Streptococcus agalactiae isolate collected from Vagina of pregnant women . All isolates were identifi ed by routine methods , automated mehods and molecular method using 16S rRNA . All isolates gave a positive test result for CAMP tset, which is considered a differential test between Streptococcus species. β-hemolytic streptococci are important human pathogens. GBS appeared decreasing in susc eptibility to penicillin with resistance ratio 15 % in Anbar city using disk diffusion agar method in the current study. Streptococcus agalactiae showed also resistant to Tetracycline (82%) , Clindamycin (60%) , Erythromycin (55%), chloramphenicol (40%) , Cipro (5%), Imipenem (0%) . Salicylic acid (SAL) reduced biofi lm formation in Streptococcus agalactiae at statistical signifi cance less than 0.001 compared with other materials . Salicylic acid exhibited synergistic effect when combination by checkerboard technique with penicillin against Streptococcus agalactiae with activity fractional inhibitory concentration index 0.41897.