Phenotypic and Genotypic Detection Ampc β-Lactmase Producing E.coli Isolated from UTI in Anbar Governate

Abstract

The plasmid facilitates the transfer of the AmpC enzyme gene to other bacteria through conjugation and transformation . Urine samples of 130 patients were collected i from both genders and i different ages of patients i with suspected urinary tract infections according to the clinical manifestations and symptoms diagnosed by the examining physician in Alramadi teaching hospital and pediatric hospital , during the period from October 2019 to February 2020 . The antibiotic susceptibility results for 9 antibiotics showed that the a highest resistance isolates to Ceftazidime (100%), Ampicilin(92%), Ceftriaxone(86%) , Trimethoprim/ Sulfamethoxazole (86%) , Cefepime(70%) ciprofloxacin (62%), Gentamicin(46%) , Cefoxitin ( 14%) and Nitrofurantoin ( 8%). Phenotypic detection for AmpC productin show that in CC-DDS test appeared 17 positive isolates , in(mCIM) method all isolate gave positive result ,EDTA use to differentiate Metallo-β-lactamase from serine cephalosporinase ,EDTA use as inhibitor of Metallo-β-lactamase ,while Modified Hodge test showed 14 isolate were positive result. The polymerase chain reaction results of twenty-three isolates showed the highest prevalence for blaFOX gene was detected in 13 isolate (56.5%), blaACC gene was detected in 12 isolate (52.1%) , bla DAH gen was detected in 12 isolate (52.1%),8 isolate (34.7% ) had blaCIT gene , bla EBC Where was identified in 6 isolate (26.08), while the lowest percentage for blaMOX gene was identified in 5 isolate (21.7%) .