Role of dimethyl sulfate on biochemical characteristics of Fragaria ananassa Duch under salinity stress in vitro

Abstract

The aims of this study were to evaluate the effect of four concentrations of Dimethylsulfate (DMS) 0%, 0.03%, 0.06% and 0.1% under four levels of salinity including 0, 30, 60 and 90 mmol NaCl L-1on some biochemistry characters such as proline, hydrogen peroxide (H2O2), catalase (CAT), superoxide dismutase (SOD), chlorophyll a (Chl a), chlorophyll b (Chlb)and total chlorophyll. All treatments were distributed according to the factorial experiment using complete ro(CRD) with five replicates. Results revealed that 0.03% of DMS gave the highest proline of 2.08 mg g-1, H2O2of 0.64 μM 100mg-1, catalase of 19.53 AU min-1g-1and SOD of 116.8 IU 100mg-1. The controlachieved the highest Chl aof35.75 mg g-1, Chl bof 23.38 mg g-1and total chlorophyll of 79.00 mg g-1. Furthermore, 30 mM NaCl L-1possesses highest proline of 2.14 mg g-1, H2O2of 0.62 μM 100mg-1, catalase of 19.38 AU min-1g-1and SOD of 114.0IU 100mg-1. The non-saline plantlets (control) gave highest Chl.a of 33.00 mg g-1, Chl b20.62 mg g-1. While 60 mmol NaCl L-1gave biggest total chlorophyll of 74.75.