Indusing some secondary metabolites from callus culture derived from Plantago psyllium and Plantago major exposed to cobalt stress.

Abstract

This experiment was conducted to study the influence of cobalt concentrations on the production of seven flavonoid compoundsin callus derived from Plantago psylliumL.and Plantago major L.Results showed that the best combination of 2,4-D and kinetin concentrationsadd to Muroshige and Skoog mediumto obtain the highest fresh weight of 541.0 mg was 3.0 and 1.0 mg.L-1 respectively. psylliumstimulated callus producedthe highest fresh weight of 365.7 mg. The addition of 75 ppm of cobalt resulted in a significantly lower fresh weight of P. psyllium callus (139.8 mg). The interaction between Plantagospecies and cobalt concentrations was significant. The callus inducted from P. majorhad significant increases of the scutallarein, apigenin, nepetinand luteolincompounds with26.40, 22.64, 14.93 and 26.20 μg.100mg-1dry weight, respectively. The production of the hispidulincompound was increased in P. psyllium at 29.40 μg.100mg-1dry weight.Also, the addition of cobalt metalstimulated the production of flavonoids at50 ppm cobalt producing the highest amounts of hispidulinand luteolinat 40.30 and 41.60 μg.100mg-1dry weight,respectively. Meanwhile, 75 ppm cobalt treatment produced the highest amount of scutallarein, apigenin, nepetinandaucubinat 25.61, 23.25, 15.90 and 13.70 μg.100mg-1dry weight, respectively.The callus inducted from P. majortreated with 50 ppm of cobalt showed the highest production of scutallarein, apigeninand luteolinat 30.33, 32.26 and 51.90 μg.100mg-1dry weight respectively. Baicaleinreached 16.46 μg.100mg-1dry weight, at 75 ppm of cobalt metal treatment in callus inducted from P. psyllium