Molecular Study Using Real Time PCR to Detect Influenza Viruses (Flu A, Flu B and RSV) in Patients at Ramadi Hospital

Abstract

Because of the increasing incidence of infections with different types of influenza of various types, type A, B, and SRV, this study included investigating the type of infection in influenza in women and men in different age groups and making a comparison between them. Methodology: 90 blood samples were collected, including 30 samples for men 30 samples for women showing clinical symptoms of infection, and 30 samples from people with clinical symptoms under control. These samples were placed in tubes containing an anticoagulant to ensure that blood does not clot DNA/RNA extraction kit, CerTest, Spanish Company and detection by Flu A, Flu B and RSV, CerTest, Spanish Company by using real time PCR. Result: Table 1 shows the investigation of some types of influenza in women who showed symptoms of infection and who are between 20 to 40 years old, where the frequency of infection in women between the ages of 20-30 years was high with types Flu A, SRV compared with control and did not show infection with type B for the same Age group . As for women aged between 30-40 years, the frequency of type B infection was high compared to control, and there were no infections with Flu a and SRV for the same age group .