EFFECT OF GOLD NANOPARTICLES WHICH EXTRACTED FROM GREEN TEA ON SOME BLOOD PARAMETERS IN MICE

Abstract

The choice of green solvent, an environmentally friendly reduction agent, in an experiment involving a synthesis of nanoparticles from the green chemistry standpoint and Non-toxic substance for nanoparticles stability Key factors. Water is in the current procedure Used worldwide as an environmentally sound solvent Polyphenols and many phytochemicals preparation and the reduction agent used in tea both nanoparticle stabilizers and, thus, robust Agglomeration shielding. Each well has been placed in RBC solution using 96-wave U-bottom Pipet 100 μl. Afterwards, for each well, 100 μl was added from every dilution of the gold nanoparticles. To triple the positive control wells add 100μl of 1 percent SDS. For negative control wells, add 100ìl of PBS. In a CO2 incubator, the incubators were 37°C for 12 hours, 5 percent (exposure time). After incubation for five minutes in 1000 rpsm, the centrifuge plate has erythrocytes intact in pellets. Draw 100 μl of supernatant from each well into a new 96-well plate. The effect of golden nanoparticles on the analysis of Red blood cells has been determined that there are no significant differtances between each concentration and the control and no significant differences exist between the same concentrations, indicating that no gold nanoparticles effect of Golden Nanoparticles has been determined by a wave-language microplate reader (FLUO star OPTIma). In comparison to control, where there is no decline in its percentage, as well as in PCV, the hemoglobin rate was found to be normal for all therapies. Compared to the control, its ratio is typical. In some therapies, there was a modest drop in the total number of white blood cells and in many other treatments.