ACTIVE COMPOUNDS ANALYSIS IN FIVE ROSELLE VARIETIES USING GC/MS

Abstract

Abstract : Field trial was laid out at private field belongings to Fallujah city 45 km west Baghdad, Iraq. Five genotypes of Roselle Hibiscus sabdariffa (malvaceae) were sown in clayey loam soil in summer during June 17, 2018. These genotypes were heet- red, heet-black, oreib, Aswan and sudan-3. Genotypes were randomly distributed within RCBD design with three replicates. GC/MS analysis was done to characterize the active compounds in calyx extract for each genotype. Results pointed that GC/MS separated 22 active compounds in heet-red calyx extract in which the highest active compound was 2, 5- pyrrolidinedione with area of 23.71% at RT of 11.708 min. In heet-black, 28 active compounds were separated with highest of 2-acetyl-5-methyl-isoxazolidin-5-ylmethyl ester of 17.24% at RT of 16.860 min, 29 active compounds with highest phenyl-1, 2- diamine of 20.55% area at RT of 12.633 min In oreib, 23 active compounds with highest 2-acetyl-5-methyl-isoxazolin-5- ylmethyl ester of 23.90% area at RT of 16.863 min. In Aswan and 15 active compounds were isolated which gave highest area of 23.19 for 2.5-pyrrolidinedione at RT of 11.682 min in sudan-3. GC/MS analysis proved that genotypes differed in number of active compounds and quality these compounds. Thus, GC/MS could be exploited to characterize the genotypes of different crops. Therefore, these genotypes could be subjected to breeding project to transfer genetic material to each other.