Study of polymorphism in the BDNF gene and its relationship to growth hormone, sodium and potassium levels in children with autism spectrum disorder in Anbar Governorate

Abstract

Autism spectrum disorder is a neurodevelopmental disorder that is usually diagnosed between (2 ‐ 6) years of age and is characterized by poor social communication, repetitive behaviors, or stereotypes, and low interest in environmental stimuli. and sensory processing or restricted interests. The current study was conducted for the purpose of studying the relationship of growth hormone and some physiological factors to polymorphism in the Brain Derived Nuetrophic Factor (BDNF) gene in a sample of 40 children with Autism spectrum Disorder (ASD) as well as a control sample of 20 children without autism spectrum disorder for the period from 1/10/2021 to 1/12/2021. The current study showed an insignificant decrease in p≤0.05 in the concentration of potassium ion in the blood serum of the study sample individuals, which amounted to (4.42 (±0.43) mg/dl) compared to its percentage in the blood serum of the members of the control sample, where it amounted to (5.09(±1.57) mg/dl.The study also indicated that there was no correlation between the concentration of growth hormone and the concentration of potassium ion between the blood serum of the study sample members as the value of the correlation coefficient (‐0.12955). The study also indicated a significant difference ≤0.05 in the concentration of sodium ion, as its concentration in the blood serum of the study sample members (123.67 (± 9.57) mmol/l compared to its concentration in the serum of the control sample members was 135.05 (± 9.43) mmol/l). It was also found that there was a significant correlation of p≤0.05 between growth hormone and the concentration of serum sodium ion in the study samples, where the value of the correlation coefficient was (0.273364). The results of the current study also show the occurrence of multiple forms or locations of nitrogen bases (SNPs), as the site rs1048221 showed the occurrence of multiplicity in the base site (A(70bp)) in which the number of beams appeared 20 (50%) in the members of the study sample compared to 18 (90%) in the individuals of the control sample, while the study showed the occurrence of multiplicity in the form of nucleotide above as it showed two additional forms, C(102) and CA (70+102). ) and the number of impressions and frequency reached 4 (10%) and 11 (36.36%) respectively compared to the number of times they appeared in the members of the control sample, which amounted to 0(0%) and 2(10%), and the occurrence of multiple in the forms or locations of nitrogen bases (SNPs), where the site rs1189205506 showed the occurrence of multiple in the base site (C(100bp)) in which the number of beams appeared 28 (70%) in the individuals of the study sample compared to 0 (0%) in the control sample, while the study showed the occurrence of multiple in the The form of nucleotides above showed two additional forms, G(87bp) and CG(100+87bp) and with the number of impressions and frequency of 4(10%) and 8(20%) respectively compared to the number of impressions in the members of the control sample, which amounted to 19 (95%) and 1(5%), as well as the occurrence of multiple forms or locations of nitrogen bases (SNPs), where the site rs1856943620 showed the occurrence of multiple in the base site (G(82bp). The number of beams appeared in it reached 11 (27.5%) in the members of the study sample compared to 18 (90%) in the control sample individuals, while the study showed the occurrence of multiple in the form of nucleotides above as it showed two additional forms, A(151bp) and GA(82+151bp) and the number of impressions and frequency of 6 (15%) and 21 (52.5%) respectively compared to the number of times they appeared in the control sample members, which amounted to 0(0%) and 2(10%).