Isolation and diagnosis of SCO-producing bacteria and improved optimal production conditions

Abstract

This study was carried out for the purpose of isolating and diagnosing oil-producing bacteria from different soil samples in Anbar province and determining the optimal conditions for production. Used for isolation amid oil dens, if 10 bacterial isolations were obtained, four of which were characterized by their ability to collect oil at a high rate and used the black sudan dye B to choose the most efficient isolation of them, namely isolation U isolated from soil planted with bamia plant, then diagnosed isolation through morphological and chemical tests and showed that it belongs to the bacteria Pseudomonas fluorescens . The results showed that corn coals were the best carbon source in oil production and that 0.1% of the nitrogen source was the best in oil production at 40.3%, The best hydrogen number at 10 was an oil production rate of 47.5%, the best lap temperature at 30 m if the oil ratio was 50.7%, and the best concentration of carbon source 2% with an oil production rate of 50.7%, The pepton was the best nitrogen source used in oil production with a 48-hour lap with a production rate of 50.7 percent.