Biosynthesis of Alumina Nanoparticles by Plant Extract for Ocimum basilicum Leaves and Evaluation of its Antibacterial Efficiency Against Some Bacteria Isolated from Skin Infections

Abstract

This study includes the isolation and identification of bacteria that cause skin infections in addition to preparing alumina nanoparticles in the biosynthesis method and testing its efficiency as an anti-bacterial. compared inhibition zones between the prepared nanoparticles and commonly used antibiotics. The prepared nanoparticles were characterized by various techniques, including UV-visible spectrophotometry, X-ray diffraction (XRD), and field emission scanning electron microscopic (FE-SEM) technology was also used to verify the morphology of the crystal structure. UV- visible spectroscopy showed success in the synthesis of alumina nanoparticles by observing the absorption peak that was (248,250) nm, which belongs to alumina nanoparticles, and X-ray diffraction confirmed the presence of Al2O3NPs in the crystal structure of the prepared particles. FE-SEM showed the formation of alumina nanoparticles in the size range of (15-63) nm and in a spherical shape. When evaluating the efficiency of alumina nanoparticles as anti-bacterial, it was found that it has a high activity by measuring the diameter of inhibition, while the aqueous plant extract did not show any inhibition diameter. Alumina nanoparticles showed high efficacy against Acinetobacter baumannii which are resistant to all types of antibiotics used in the current study. The antibiotics Imipenem (IPM), amikacin (AK), Ciprofloxacin (CIP), Gentamycin (GM) were used to test their effect on gram-negative bacteria while Imipenem (IPM), amikacin (AK), Vancomycin (VA), Amoxicillin/clavulanic acid (AMC) on gram-positive bacteria, where some bacterial isolates were resistant and others sensitive to these antibiotics.