The Effect of Alcoholic and Aqueous extract of Capparis spinosa leaves on histological and physiological structure in the liver of male rats injected with lead acetate intraperitoneally

Abstract

The current study aimed to investigate the curative and preventive effect of alcoholic and aqueous extract of Capparis spinosa leaves on the histological structure of the kidneys and some biochemical parameters of male rats injected intraperitoneally with lead acetate. The biochemical parameters included estimating the level of liver enzymes: GGT, ALP, AST, ALT and total antioxidants (T-AOX), glutathione (GSH) and malondial aldehyde (MDA) assay. The study included 40 male laboratory male albino rats of the Sprague dawley breed, with weights ranging between 170-200 grams, and they were randomly divided into eight groups. The results showed that the peritoneal injection of male rats with lead acetate caused a significant increase at the level of probability ((P≤0.05) in the level of liver enzymes GGT, ALP, AST, ALT and Malone Dialdehyde (MDA), while there was a significant decrease (P≤0.05( in the level of antigens. Total oxidative stress (T-AOX) and glutathione (GSH) in the blood serum compared with the control group The results of groups that were injected with lead acetate intraperitoneally for 30 days and then dosed with alcoholic extract of caper leaves for another 30 days as well as groups that were first dosed with alcoholic extract and aqueous extract showed For caper leaves and after 3 hours, britonia injected with lead acetate showed a significant decrease at the level of probability ((P≤0.05) in the level of liver enzymes GGT, ALP, AST, ALT and malon dialdehyde (MDA) compared with the group that injected Britonia with lead acetate, while the results showed Significant increase in total antioxidants (T-AOX) and glutathione (GSH) compared with the group that was injected with lead acetate intraperitoneally.The results of the study for groups that were dosed with alcoholic extract of caper leaves only showed no significant changes at the level of Tally (P≤0.05) in the level of GGT, ALP, AST, ALT, Malone Dialdehyde (MDA) and total antioxidants (T-AOX) compared with the control group, while the groups dosed with aqueous extract of caper leaves did not show any significant changes at the level of Probability ((P≤0.05) in any of the studied variables compared with the control group. The current study also showed significant histological changes in the liver tissue in the group that injected Britonia with lead acetate, while the groups treated with alcoholic and aqueous extract of capers leaves showed an improvement in the histological picture of the liver tissues.