Bioremediation of Chlorpyrifos Insecticide by using Aeromonas Hydrophila Bacteria

Abstract

Several bacteria species may have the ability to degrade and digest soundly the residuals of certain insecticide with causing any deteriorated consequences. This work was designed to examine the ability of Aeromonas hydrophila bacteria for biodegrading of chlorpyrifos insecticide from contaminated soil. However, soil sample was collected from home garden at depth of 10 cm while the chlorpyrifos insecticide was obtained from the local market. Meanwhile Aeromonas hydrophila bacteria were isolated from the garden soil. The bacterial cultural media were prepared to consist of (NH4)2SO4 (1g), NaCl (6g ), K2HPO4 (0.5g), yeast extract (1g ), MgSO4 (0.1g) and CMC (5g). This work has examined the efficiency of A. hydrophila bacteria to remove insecticide from the soil under various growing conditions such as five different pH values, five termperature degrees and four insecticide concentrations. The obtained results showed that the highest bacterial efficiency (98.0 ± 7.8%) was recorded as pH value of 6 and it was 91.1 ± 6.0% at temperature of 40 ° C while it was 99.1 ± 6.8 % at insecticide concentration of 25 ppm.