THE EFFECT OF Lepidium Sativum SEEDS EXTRACT ON SOME OXIDATIVE STRESS, ANTIOXIDANTS AND HISTOLOGICAL CHANGES IN RAT TREATED WITH CCL4

Abstract

The aim of the current study is to the investigate the effect of hepatoprotective and antioxidant of seeds extracts of the Lepidium Sativum extract against liver damage induced by carbon tetrachloride. In our study, white rats were used. CCl4 (0.1) ml \100 gram body weight use to inject the rats intrapersonal mixed in olive oil same amount, twice a week for twelve weeks and treated orally with (LSS) (200 mg/kg) daily for twelve weeks and compared with a group of rats injected intrapersonal with CCl4 (0.1ml\100g b.w.) mixed in olive oil same amount, twice a week for twelve weeks as a control group. CCl4 administration is caused significant changed in serum enzymes (ALP GOT and GPT), oxidant substances (MDA, CAT, GSH and SOD) indicative of hepatic injury. The results revealed that the (LSS) extract significantly decreased AST, ALT and ALP levels. The antioxidant parameters GSH, GPx, SOD and catalase levels were increased considerably compared to their levels in groups not treated with (LSS). Histopathological findings revealed that liver of CCl4-treated rats showed Degeneration, thickness wall of the central vein; the inflammatory cells are infiltrating into portal areas and central vein. There are ameliorated significantly in the rat's groups, that treated by LSS result in a decrease in degenerated hepatocytes and there are reduce in necrosis in the group that treated by LSS (200) mg/kg followed by CCl4 administration, and demonstrated less nuclear degeneration as compared to CCl4 group. Treatment of LSS (200) mg/kg and then followed by CCl4 injection results in a decrease in nuclear degeneration. It could conclude, LSS extract has a protective effect against CCl4 toxicity. Our results are very promising LSS extract could be used to protect liver tissues against CCl4.