Efficacy Of Green Synthesis Of Silver Nanoparticles Using Allium Ampeloprasum Against Isoproterenol Induced Myocardial Infarction In Adult Albino Rats.

Abstract

Main goal of study was to see if Allium ampeloprasum (Leek) aqueous extract and silver nanoparticles (AgNPs) biosynthesized by this extract could protect male Wistar rats from cardiototoxicity produced by isoprenaline (ISO). The contents of Leek aqueous extract were also discovered, as well as biosynthesized AgNPs. Thirty mature Wistar albino rats of (male and female) randomly assigned to six category. In group I dosed with distilled water (5 ml / kg) and then injected subcutaneously with normal saline local solution at a concentration (0.1 ml / 100 g) on days 44 and 45 from the start of the experiment. The rats in group II were given ISO subcutaneous injection 65 mg/kg for two days on days 44 and 45 after the trial began. In group III Pre-treated dosed with silver nanoparticles prepared from leek extract (AgNPS) for 43 days and on days 44 and 45 injected subcutaneously with ISO (65 milligrams / kilogram) for two consecutive days from the start of the experiment only, while in Group IV Pre-treated with aqueous extract of Leek at a concentration of 200 mg/kg every day for 43 days, and on days 44 and 45, (ISO) was injected subcutaneously (65 milligrams / kilogram) for two consecutive days from the start of the experiment only. In Group V Dosed with silver nanoparticles prepared from Leek extract at a concentration of 200 milligrams / kilogram for 45 days, while in group VI was orally dosed with aqueous extract of Leek at a concentration of 200 milligrams / kilogram for 45 days and MI was established. The cardioprotective effects of Leek extract and AgNPs/Leek extract were demonstrated by a reduction in high serum concentration of heart damage enzymes (CK-MB, ALT, AST, and LDH), as well as an increase in heart bioindicators of oxidative stress (MDA) and scavenger deficiency ( GSH , SOD and CAT )AgNPs/Leek extract was more efficient than Leek extract in terms of cardiac histological alterations In summation, Leek extract and AgNPs/Leek extract, which is more effective, may protect Wistar rats against ISOinduced cardiotoxicity, and this effect may be mediated, at least in part, by antioxidant defense system augmentation.