EFFICACY OF LEPIDIUM SATIVUM SEEDS AGAINST CARBON TETRA CHLORIDE INDUCED HEPATOTOXICITY IN RATS

Abstract

: The aim is to assess the hepatoprotective and antioxidant effect of Lepidium sativum extract against different liver damage caused by carbon tetrachloride. We used white rats in this study. The included rats were divided into two groups, the case group and the control group. We injected the rats of the case group with 0.1 ml of CCl4 per 100 gram body weight mixed with the same amount of olive oil intraperitoneally two times weekly and treated them with (LSS) in a dose of 200 mg/kg daily for twelve weeks. The rats in the control group were injected with 0.1 ml of CCl4 per 100 gram body weight mixed with the same amount of olive oil intraperitoneally two times weekly for twelve weeks. CCl4 administration resulted in marked changes in the serum levels of liver enzymes, including (GOT, GPT and ALP) and oxidant substances, including (MDA and CAT), which indicates that it caused liver injury. In addition, our results showed that LSS extract administration resulted in a marked decrease inthe GOT, GPT and ALP levels and increase in the antioxidants, including glutathione peroxidase, catalase and reduced glutathione levels in the treated group compared to control group. In addition, the results of histopathological examination of the liver showed degenerated liver cells, thickened central vein walls and the presence of inflammatory cells in the portal tracts and central veins in the ratsin the control group. However, these changes improved markedly in those of the case group which received LSS, where they showed reduction in the degeneration and necrosis of the liver cells. This means that treatment with LSS in a dose of 200 mg/kg and followed by CCl4 injection protects against its nuclear degeneration effect. We conclude that LSS extract has a protective role against CCl4 toxic effect. So, it can be used as a prophylactic agent against variantliver injuries induced by CCl4