Transformation of some genetic traits carried on Staphylococcus aureus plasmids to E. coli BL21(DE3) strain

Abstract

This study included identified some of the genetic traits carried on plasmids for Staphylococcus aureus isolated from urinary infections cases. The test of antibiotic resistance appears the isolated bacteria was resistance for each of the (AM, CIP, MET, TMP, B, RA) and sensitive for (TOB, C, TE), also it was beta heamolytic on blood agar and able to lysis of casein protein. The electrophoresis showed three bands of S. aureus DNA plasmids. The isolated plasmids used in the transformation process to E. coli BL21(DE3) strain, this strain sensitive for all antibiotic used in this study except (RA) and it was not able to lysis of blood and casein protein. The result of transformation showed ability of transformantE.coli to produce the heamolysin and became resistance for (AM, MET, TMP, B) but it was not lysis of casein. The transformantE. coli has same plasmid pattern for S. aureus.