EFFECT OF DIETARY NATURAL FEED ADDITIVES TO MINIMIZE NEGATIVE ROLE OF PEROXIDE HYDROGEN IN BROILER

Abstract

This study was conducted in poultry farm belong to Animal Resources and Fisheries Research center, Ministry of Sciences and Technology, from 21/11/2018 to 2/1/2019 for 42 day. The aim of study was to find out effect of Green tea and Organic Selenium, as antioxidant and their effects on immunity response and oxidation parameters of broiler. One Hundred – Ninety Eight of males broiler (Ross 308) chicks with average initial body weight of 42 gm./ chick were used in this study. Chicks were randomly assigned to six treatments, each treatment was sub-divided into three replicates (11 chicks per replicate). The treatments were as follows: T1 basal diet free from any addition (Negative control), T2 included addition of 0.5% H2O2 to drinking water at the age 10 days (Positive control), T3 basal diet + 1.5% green tea to feed, T4 basal diet + 1 mg/kg Organic Selenium to feed, T5 basal diet supplemented with 1.5% green tea + H2O2 (0.50%), T6 basal diet supplemented with 1 mg/kg Organic Selenium + H2O2 (0.50%). The results revealed significant differences between treatments in (HI) titer against Newcastle and Gumboro diseases. However, T2 (Negative control) recorded lowest values with significant difference with other treatments. Also, no significant differences were observed by adding green tea and organic selenium on relative weights of Bursa, while relative weights of spleen and thymus recorded significant differences between treatments and T5 showed significant increase as compared with other treatments, while Highly significant (P<0.01) observed for T6 in relative weight of thymus and recorded 0.370. Results included significant differences between treatments in Creatinine and Uric acid. T2 showed a significant increase in Malondialdehyde (MDA), Peroxide Value (P.V) and Free Fatty Acids (FFA) in liver tissue as compared with other treatments. On other hand, feed additives treatments showed improvement in liver tissues content of Glutathione peroxidase and catalase as compared with T2 (Negative control). As well as, T1 (positive control) has highly significant (P<0.01) in Glutathione peroxidase and catalase enzymes.