DETECTION OF TRICHOTHECENE TOXIN BY HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY(HPLC) ANDTHIN LAYER CHROMATOGRAPHY TEST (TLC) TECHNOLOGY OF GRAIN CONTAMINATED WITH FUSARIUMGRAMINEARUM FUNGI

Abstract

The current study aimed using several techniques in detecting some isolates of the Fusariumgraminearumfungi, testing their ability to produce or not producing tricothecencs toxin, and the techniques used in this study like the technique of High-performance liquid chromatography (HPLC) and Thin Layer Chromatography test (TLC) technology. Isolates eight isolates of different origin and source, isolating one clinical isolation (cow’s lung) and seven environmental isolates (wheat and barley),These fungi were cultured on Potato dextrose agar (PDA) and then sub cultured on Sabouraud dextrose agar (SDA). The isolates were identified depending on their morphological characteristics (cultural and microscopical). The results showed that the color of the Fusarium colony was initially cotton us but soon became white in the anterior and zebra direction in the opposite direction, while its shape was similar to the large banana in microscopic tests. The study showed no differences in the results of the technique of liquid chromatography and chromatography test. This is a confirmation of the findings of the present study with other studies. If isolates were found to have the ability to produce poison and the highest percentage of semolina in barley samples was (18.72 - 18.75) compared to wheat samples Which was observed to be unable to produce the poison and this indicates the existence of significant differences between the isolates of wheat and barley in terms of their origins, while no significant differences between the isolates in terms of origin (clinical, environmental). As for the test of accurate color separation was observed discoloration of the same samples producing the poison Boas Of technical liquid high chromatography test performance by comparing it with standard, and the reason is due to the ability of some isolates the toxin production and other do not have the ability to produce poison for the absence of the gene responsible for the production of this poison or mutation genetic led to his absence in the other isolates.