Effect of Tryptophan injection on the semen quality in Iraqi Shami buck

Abstract

Abstract The aim of this study was to determine the effects of tryptophan injection on semen quality of Shami buck. Animals were divided randomly into four equal groups (3 for each group). Tryptophan was injected in the muscle and semen collected twice weekly by Artificial vagina. The first group (T1) left without treated as control group, the second group (T2) was injection Tryptophan 0.1 g, the third group (T3) was injection Tryptophan 0.125 g and the fourth group (T4) was injection Tryptophan 0.150 g. Semen was collected from twelve Shami buck, 2 ejaculates each, by an Artificial vagina. Semen were evaluated after collected for Volume (ml), Mass and Individual motility (%), sperm concentrations ×109, Live and dead sperm (%) and Sperm abnormality (%). Blood samples were collected via vacationer tubes (5 ml) from jugular vein to analyzed of testosterone hormone. Results showed that tryptophan injection of semen ejaculation was significantly (P<0.05) in T4 and T3. The percentage of Mass activity, Individual motility, live sperm and sperm concentration were significantly increased in the T4, T3 and T2 significantly (P<0.05). The percentage of dead sperm and sperm abnormality were significantly increased (P<0.05) in treatments T1 and T2. The testosterone concentration was significantly higher in the T4 (P<0.05). In conclusion, 0.150 g improved tryptophan injection and testosterone level in Shami buck.