Early Genetic Detection of Cyclospora Cayetanensis that Causes Diarrhea in Children at the Children's and Gynecology Hospital in Ramadi – Iraq

Abstract

This study was conducted to find out the prevalence of Cyclosporiasis in children arriving at the Children's and Gynecology Hospital in Ramadi – Iraq , Because this parasite has a significant impact on causing dehydration and the loss of large amounts of body fluids, and consequently the confirmed death of the infected person if not treated within the specified period, as well as the identification of the means of transmission to humans and our results showed its transmission through water and food contaminated with C. cayetanensisOocysts , and it was used. Simple and modern methods of diagnosing infect . This study included examining (250) samples of faeces from children under the age of 15 years from the period 22-7-2020 to 28-2-2021 and those suffering from diarrhea who visited Children's and Gynecology Hospital since July of 2020. The results showed that the percentage of infection prevalence in children for all pathological parasites is (23.6%) and the number reached 59 infected samples, while the percentage of infection with the Cyclospora was 6.4% (according to microscopic diagnosis using modified Ziehl–Neelsen stain, sedimentation and sedimentation methods, and direct method. , Where the infection rate of Entamoeba histolytica (10.4), Giardialambelia (1.60), Cryptosporidiumparvum(3.20), Cyclosporacayetanensis (6.4), Trichomonas hominis (0.4) and Entamoeba coli (1.60), and the prevalence of infection in children according to the genetic diagnosis of the Cyclospora sample were (4.0%), and this was the first study in Anbar Governorate to prove the presence of this parasite.