PROBIOTIC ANTAGONISTIC EFFECTS OF Lactobacillus collinoides FROM COW MILK ON COW-DIARRHEA-RECOVERED ENTEROTOXIGENIC E. coli: AN IN VIVO STUDY

Abstract

Probiotics have been recorded to introduce vital changes in the functions of various body organs of humans or animals. The current in vivo study was launched to unveil the antagonistic effects of Lactobacillus collinoides, lactic acid bacteria, isolated from cow milk on the induced infection by enterotoxigenic Escherichia coli (ETEC) in mice. Twenty-four albino mice (Balb/C) were divided randomly into six groups (four animals/group) designated as a negative control (NC) group which received nothing, anETEC-infected (EE) group at 0.1ml of 105 cfu/ml/orally for two days (positive control), a L. collinoides exposed (LC) group at 0.1ml of 1010cfu/ml/orally for two days, and an ETEC-exposed, at 0.1ml of 105 cfu/ml, pre-treaded with L. collinoides at 0.1ml of 1010cfu/ml/orally for two days (EE+LC) group.After the experiment period was done, blood samples were collected from all animals for understanding liver functions via glutamic oxaloacetic transaminase (GOT) and glutamate-pyruvate transaminase (GPT) tests.Then, the mice were sacrificed (10 days after the last dose received by the animals) for doing histopathological examination that targeted the liver, intestine, and stomach. When compared with the NC group, significant (p<0.05) increases in the serum levels of GOT and GPT were seen in the EE group induced after the ETEC infection. For the LC group and when compared to the NC results of GOT and GPT, significant (p<0.05) decreases in the serum levels of these enzymes were noticed in the LC group after the insertion of L collinoides. However, dosing animals of the EE+LC group with L. collinoides then inducing infection with ETEC revealed significant (p<0.05) decreases in the serum GOT and GPT levels in comparison to those in EE group with no significant (p˃0.05)differences in those levels from the EE+LC group when compared to those in the NC group. The histopathological findings demonstrated that ETEC caused necrosis, degeneration, and infiltration of inflammatory cells in the tested organ tissues; however, dosing EE+LC animals with L. collinoides protected those organ tissues from the cytotoxic effects of the ETEC. This study indicates powerful effects of the Lactobacilluscollinoides against the induced infection by enterotoxigenic E. coli.